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bone resorption test kit  (Cosmo Bio USA)

 
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    Structured Review

    Cosmo Bio USA bone resorption test kit
    Inhibitory effect on osteoclast differentiation and bone <t>resorption.</t> (A) RAW264.7 cells were cultured in DMEM high glucose for 5 days in the presence of RANKL (50 ng/mL) with CBD, taurine, and both. (B) RAW264.7 cells were seeded in CaP-coated 48-well plates and treated with 50 ng/mL RANKL in the presence of CBD, taurine, and both. (C) TRAP-positive multinucleated cells (TRAP+ MNCs) were counted after fixation and staining for TRAP. (D) Pit area was measured. The results are presented as means ± SD (n=3). *** p <0.001 indicates significant differences from CBD+taurine treated groups with CBD and taurine alone.
    Bone Resorption Test Kit, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bone+resorption+assay+kit+48/pmc11704405-63-10-16
    Average 94 stars, based on 1 article reviews
    bone resorption test kit - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Combination of Cannabidiol with Taurine Synergistically Treated Periodontitis in Rats"

    Article Title: Combination of Cannabidiol with Taurine Synergistically Treated Periodontitis in Rats

    Journal: Biomolecules & Therapeutics

    doi: 10.4062/biomolther.2024.104

    Inhibitory effect on osteoclast differentiation and bone resorption. (A) RAW264.7 cells were cultured in DMEM high glucose for 5 days in the presence of RANKL (50 ng/mL) with CBD, taurine, and both. (B) RAW264.7 cells were seeded in CaP-coated 48-well plates and treated with 50 ng/mL RANKL in the presence of CBD, taurine, and both. (C) TRAP-positive multinucleated cells (TRAP+ MNCs) were counted after fixation and staining for TRAP. (D) Pit area was measured. The results are presented as means ± SD (n=3). *** p <0.001 indicates significant differences from CBD+taurine treated groups with CBD and taurine alone.
    Figure Legend Snippet: Inhibitory effect on osteoclast differentiation and bone resorption. (A) RAW264.7 cells were cultured in DMEM high glucose for 5 days in the presence of RANKL (50 ng/mL) with CBD, taurine, and both. (B) RAW264.7 cells were seeded in CaP-coated 48-well plates and treated with 50 ng/mL RANKL in the presence of CBD, taurine, and both. (C) TRAP-positive multinucleated cells (TRAP+ MNCs) were counted after fixation and staining for TRAP. (D) Pit area was measured. The results are presented as means ± SD (n=3). *** p <0.001 indicates significant differences from CBD+taurine treated groups with CBD and taurine alone.

    Techniques Used: Cell Culture, Staining

    Related Articles

    Activity Assay:

    Article Title: Effects of Propofol on the Formation and Activity of Osteoclast-Like Cells
    Article Snippet: After treating the membrane with Peroxidase-labeled Streptavidin®, the target proteins were detected using the chemiluminescent reagent ClarityTM Western ECL Substrate (Bio-Rad Laboratories). .. Mineral resorption activity and pit formation assay RAW264.7 cells were seeded into calcium phosphate-coated plates included in Bone Resorption Assay Kit 48® (Cosmo Bio Co., Ltd.) and cultured for 4 days under the conditions described above. .. After removing the cells by treating the plates with 5% sodium hypochlorite, the formation of pits was examined using an ECLIPSE TS2R inverted microscope (Nikon Corp., Tokyo, Japan).

    Tube Formation Assay:

    Article Title: Effects of Propofol on the Formation and Activity of Osteoclast-Like Cells
    Article Snippet: After treating the membrane with Peroxidase-labeled Streptavidin®, the target proteins were detected using the chemiluminescent reagent ClarityTM Western ECL Substrate (Bio-Rad Laboratories). .. Mineral resorption activity and pit formation assay RAW264.7 cells were seeded into calcium phosphate-coated plates included in Bone Resorption Assay Kit 48® (Cosmo Bio Co., Ltd.) and cultured for 4 days under the conditions described above. .. After removing the cells by treating the plates with 5% sodium hypochlorite, the formation of pits was examined using an ECLIPSE TS2R inverted microscope (Nikon Corp., Tokyo, Japan).

    Cell Culture:

    Article Title: Effects of Propofol on the Formation and Activity of Osteoclast-Like Cells
    Article Snippet: After treating the membrane with Peroxidase-labeled Streptavidin®, the target proteins were detected using the chemiluminescent reagent ClarityTM Western ECL Substrate (Bio-Rad Laboratories). .. Mineral resorption activity and pit formation assay RAW264.7 cells were seeded into calcium phosphate-coated plates included in Bone Resorption Assay Kit 48® (Cosmo Bio Co., Ltd.) and cultured for 4 days under the conditions described above. .. After removing the cells by treating the plates with 5% sodium hypochlorite, the formation of pits was examined using an ECLIPSE TS2R inverted microscope (Nikon Corp., Tokyo, Japan).



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    Image Search Results


    Inhibitory effect on osteoclast differentiation and bone resorption. (A) RAW264.7 cells were cultured in DMEM high glucose for 5 days in the presence of RANKL (50 ng/mL) with CBD, taurine, and both. (B) RAW264.7 cells were seeded in CaP-coated 48-well plates and treated with 50 ng/mL RANKL in the presence of CBD, taurine, and both. (C) TRAP-positive multinucleated cells (TRAP+ MNCs) were counted after fixation and staining for TRAP. (D) Pit area was measured. The results are presented as means ± SD (n=3). *** p <0.001 indicates significant differences from CBD+taurine treated groups with CBD and taurine alone.

    Journal: Biomolecules & Therapeutics

    Article Title: Combination of Cannabidiol with Taurine Synergistically Treated Periodontitis in Rats

    doi: 10.4062/biomolther.2024.104

    Figure Lengend Snippet: Inhibitory effect on osteoclast differentiation and bone resorption. (A) RAW264.7 cells were cultured in DMEM high glucose for 5 days in the presence of RANKL (50 ng/mL) with CBD, taurine, and both. (B) RAW264.7 cells were seeded in CaP-coated 48-well plates and treated with 50 ng/mL RANKL in the presence of CBD, taurine, and both. (C) TRAP-positive multinucleated cells (TRAP+ MNCs) were counted after fixation and staining for TRAP. (D) Pit area was measured. The results are presented as means ± SD (n=3). *** p <0.001 indicates significant differences from CBD+taurine treated groups with CBD and taurine alone.

    Article Snippet: The bone resorption pit assay was carried out using a Bone Resorption Test Kit (CSF-BRA-48 KIT, Cosmo Bio) in plates coated with calcium phosphate.

    Techniques: Cell Culture, Staining